猪SOCS4基因的克隆、生物信息学分析及热应激条件下的表达

doi:10.11843/j.issn.0366-6964.2015.02.021

Abstract

Abstract:

The aim of this study was to systematically elucidate the mechanism of heat stress-induced swine immunosuppression，and inflammatory factors disorder.The TZ (Luchuan pig×Landrace pig)porcine SOCS4 gene was cloned in this study，it consisted of 1 326 bp of ORF encoding a protein with 441 amino acids，285 amino acids of them formed the N-terminal of SOCS4.It shared high amino acid sequence identity with that in Landrace pigs(100.0%)，Bos Taurus(96.1%)，Capra Hircus(96.1%)，Felis catus (95.2%)，Equus caballus(94.6%)，Canis lupus familiaris (94.3%)，Homo sapiens (93.0%) and Mus musculus (88.1%).The molecular weight of SOCS4 protein was 50.5 ku，and PI was 6.60.qRT-PCR results showed that the expression of SOCS4 in thymus gland of TZ pigs was significantly upregulated on day 3 and 9 after heat stress began (P≤0.05).As well in mesenteric lymph node，where it significantly upregulated on day 1 and 6 (P≤0.01).The expression of SOCS4 decreased significantly in small intestine and spleen of TZ pigs during heat stress (P≤0.05).The TZ porcine SOCS4 cDNA was cloned successfully，and a significant changes of SOCS4 mRNA expression were appeared in heat stressed pigs with a tissue and time dependence manner.

CLC Number:

S858.31

WANG Ping，JU Xiang-hong，YONG Yan-hong，JIA Ru-min，MA Ming-long，ZHAO Yun-tao，LIAO Ming. Cloning and Bioinformatics Analysis of Porcine SOCS4 cDNA and Its Dynamic during Heat Stress[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 323-331.

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Cloning and Bioinformatics Analysis of Porcine SOCS4 cDNA and Its Dynamic during Heat Stress

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